Scenario:
You are about to receive the Mad Scientist Award from HCJC for your pioneering work on the Sick Cow Syndrome, which costs dairies worldwide billions of dollars in terms of losses.
In the course of your research, you managed to isolate the cDNA sequence of the gene that you believe is responsible for the disease. Note that the cDNA sequence reverse-transcribed from mRNA the first cDNA strand is complementary to the mRNA strand. The first cDNA strand is then used as a template in DNA replication to obtain the double-stranded cDNA
Armed with your knowledge of Bioinformatics, you sought to learn more about the gene.
The nucleotide sequence (non-transcribing strand) of the cDNA is as follows:
1 tgcattcgag gctgccagca agcaggtcct cgcagccccg ccatggccct gtggacacgc
61 ctgcggcccc tgctggccct gctggcgctc tggccccccc ccccggcccg cgccttcgtc
121 aaccagcatc tgtgtggctc ccacctggtg gaggcgctgt acctggtgtg cggagagcgc
181 ggcttcttct acacgcccaa ggcccgccgg gaggtggagg gcccgcaggt gggggcgctg
241 gagctggccg gaggcccggg cgcgggcggc ctggaggggc ccccgcagaa gcgtggcatc
301 gtggagcagt gctgtgccag cgtctgctcg ctctaccagc tggagaacta ctgtaactag
361 gcctgccccg acacaataaa cccttacgag caag
To begin with, you decide to translate the nucleotide sequence into meaningful polypeptide sequence. Using the ExPASy Molecular Biology Server (http://us.expasy.org/), translate the nucleotide sequence into polypeptide sequence using the translate tool from the Tools and Software Package.
You have six possible reading frames for the gene. Which is the correct reading frame (i.e. one that will yield a meaningful sequence of amino acids)?
You are about to receive the Mad Scientist Award from HCJC for your pioneering work on the Sick Cow Syndrome, which costs dairies worldwide billions of dollars in terms of losses.
In the course of your research, you managed to isolate the cDNA sequence of the gene that you believe is responsible for the disease. Note that the cDNA sequence reverse-transcribed from mRNA the first cDNA strand is complementary to the mRNA strand. The first cDNA strand is then used as a template in DNA replication to obtain the double-stranded cDNA
Armed with your knowledge of Bioinformatics, you sought to learn more about the gene.
The nucleotide sequence (non-transcribing strand) of the cDNA is as follows:
1 tgcattcgag gctgccagca agcaggtcct cgcagccccg ccatggccct gtggacacgc
61 ctgcggcccc tgctggccct gctggcgctc tggccccccc ccccggcccg cgccttcgtc
121 aaccagcatc tgtgtggctc ccacctggtg gaggcgctgt acctggtgtg cggagagcgc
181 ggcttcttct acacgcccaa ggcccgccgg gaggtggagg gcccgcaggt gggggcgctg
241 gagctggccg gaggcccggg cgcgggcggc ctggaggggc ccccgcagaa gcgtggcatc
301 gtggagcagt gctgtgccag cgtctgctcg ctctaccagc tggagaacta ctgtaactag
361 gcctgccccg acacaataaa cccttacgag caag
To begin with, you decide to translate the nucleotide sequence into meaningful polypeptide sequence. Using the ExPASy Molecular Biology Server (http://us.expasy.org/), translate the nucleotide sequence into polypeptide sequence using the translate tool from the Tools and Software Package.
You have six possible reading frames for the gene. Which is the correct reading frame (i.e. one that will yield a meaningful sequence of amino acids)?